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TGF alpha Antibody

CAT: 0800-V2267-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V2267-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TGF alpha is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human
Immunogen
A 10-amino acid synthetic peptide (aa 34-43) from human TGF alpha was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgM k
Clone
1E8-G6
Applications
FACS, IF, IHC-P
Purity
PEG precipitation
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the TGF alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TGF alpha antibody is available for research use only.
Storage Conditions
Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Location
Cytoplasmic and secreted
Image Legend
Formalin-fixed, paraffin-embedded human testicular carcinoma stained with TGF-alpha antibody (1E8-G6)

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