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TGF alpha Antibody

CAT: 0800-V2266-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V2266-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TGF alpha is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.
CAS Number
9007-83-4
Specifications
ELISA: 2-4 µg/mL (order BSA/azide-free format), Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
MF9
Applications
ELISA, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the TGF alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TGF alpha antibody is available for research use only.
Storage Conditions
Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
Location
Cytoplasmic and secreted
Image Legend
IHC testing of FFPE human testicular carcinoma with TGF alpha antibody
Product Datasheet
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