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Podocalyxin Antibody

CAT: 0800-V2227IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V2227IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Host
Mouse
Reactivity
Human, Rat
Immunogen
A human recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain was used as the immunogen for the Podocalyxin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
3D3
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the Podocalyxin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Podocalyxin antibody is available for research use only.
Storage Conditions
Store the Podocalyxin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Podocalyxin antibody to be titered up or down for optimal performance.
Location
Cell surface and cytoplasmic
Image Legend
IHC staining of FFPE human placenta with Podocalyxin antibody (clone 3D3) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

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