CD11b Antibody / MAC-1 / ITGAM

• Description:

  CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin alpha-M (CD11b) combines with the Integrin beta-2 (CD18) to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3) . Integrin alpha-M/beta-2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Functional studies (order BSA/sodium azide-free format)

• Host:

  Rat

• Reactivity:

  Human, Mouse

• Immunogen:

  B10 mouse spleen cells enriched for T lymphocytes were used as the immunogen for this CD11b antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  M1/70

• Applications:

  FACS, IF, Functional studies

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the CD11b antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD11b antibody is available for research use only.

• Storage Conditions:

  Store the CD11b antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD11b antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasm

• Image Legend:

  SDS-PAGE Analysis of Purified, BSA-Free CD11b Antibody (clone M1/70) . Confirmation of Integrity and Purity of the Antibody.