Ku70 + Ku80 Antibody

• Description:

  This antibody recognizes a dimer of two proteins of 70kDa (Ku70) and ~80kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence/Immunocytochemistry: 1-2 µg/mL for 30 min at RT

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  KU729

• Applications:

  FACS, IF/ICC

• Purity:

  Protein G purified monoclonal antibody

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the Ku70 + Ku80 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Ku70 + Ku80 antibody is available for research use only.

• Storage Conditions:

  Store the Ku70 + Ku80 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  FACS testing of permeabilized human 293T cells with Ku70 + Ku80 antibody. Black=cells alone; Gray=isotype control; Green= AF488 conjugated Ku70 + Ku80 antibody.