Ku70 + Ku80 Antibody PE Conjugate

• Description:

  This antibody recognizes a dimer of two proteins of 70kDa (Ku70) and ~80kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

• Specifications:

  Flow Cytometry : 5ul/test/million cells or 5ul/test/100ul of whole blood, Immunofluorescence : 1:50-1:100 for 30 minutes at RT

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  KU729

• Applications:

  FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  PE Conjugate

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the Ku70 + Ku80 antibody at 2-8oC. Conjugate is light sensitive, store in the dark.

• Limitations:

  This Ku70 + Ku80 antibody is available for research use only.

• Storage Conditions:

  Store the Ku70 + Ku80 antibody at 2-8°C. Conjugate is light sensitive, store in the dark.

• Formulation:

  500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  FACS testing of human K562 cells with Ku70 + Ku80 antibody. Black=cells alone; Green=isotype control; Red= PE conjugated Ku70 + Ku80 antibody.