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Melan-A Antibody

CAT: 0800-V2117IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V2117IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human Melan-A protein was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
M2-9E3
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the Melan-A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Melan-A antibody is available for research use only.
Storage Conditions
Store the Melan-A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Cytoplasmic
Image Legend
Formalin/paraffin human melanoma stained with MART-1 / Melan-A antibody (clone M2-9E3) . Note cytoplasmic staining of cells.
Product Datasheet
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