Estrogen Receptor beta Antibody

• Description:

  Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.

• Specifications:

  Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood

• UniProt:

  Q92731

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  The C-terminus fragment of recombinant human Estrogen Receptor beta protein was used as the immunogen for this antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  ERb455

• Applications:

  FACS

• Purity:

  Protein G affinity chromatography

• Format:

  CF488 Conjugate

• Buffer:

  500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the Estrogen Receptor beta antibody at 2-8oC, protected from light.

• Limitations:

  This Estrogen Receptor beta antibody is available for research use only.

• Storage Conditions:

  Store the Estrogen Receptor beta antibody at 2-8°C, protected from light.

• Formulation:

  500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Estrogen Receptor beta antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  FACS testing of human BT474 cells with Estrogen Receptor beta antibody: Black=cells alone; Gray=isotype control; Green=Estrogen Receptor beta antibody.