Estrogen Receptor beta Antibody

• Description:

  Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including alpha and beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER alpha and beta are differentially activated by various ligands. Ligand interaction triggers a cascade of events, including dissociation from heat shock proteins, recepter dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER alpha and beta may be regulated by distinct mechanisms even though they share many functional characteristics.

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  The C-terminus fragment of recombinant human Estrogen Receptor beta protein was used as the immunogen for this antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  NR3Gb-1

• Applications:

  IHC

• Purity:

  Protein G purified

• Format:

  Purified

• Buffer:

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution:

  Store the Estrogen receptor beta antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Estrogen Receptor beta antibody is available for research use only.

• Storage Conditions:

  Store the Estrogen receptor beta antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number:

  9007-83-4

• Location:

  Predominantly nuclear

• Image Legend:

  IHC testing of ovarian cancer stained with Estrogen Receptor beta antibody (clone NR3Gb-1) .