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P27Kip1 Antibody

CAT: 0800-V2086IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V2086IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. The p27Kip1 antibody is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. It is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Purified GST-p27 fusion protein of human origin was used as the immunogen for this p27Kip1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SX53G8
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the p27Kip1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This p27Kip1 antibody is available for research use only.
Storage Conditions
Store the p27Kip1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Nuclear
Image Legend
IHC staining of FFPE human prostate carcinoma tissue with p287Kip1 antibody (clone SX53G8) . HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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