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CD79a Antibody Cocktail

CAT: 0800-V2080-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V2080-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. CD79a antibody is generally used to complement CD20 antibody especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20) . This antibody cocktail will stain many of the same lymphomas as CD20 antibody, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is CD20 antibody. CD79a antibody also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to aa 202-216 (GTYQDVGSLNIADVQ) of human CD79a protein (JCB117 and HM47/A9) was used as the immunogen for this antibody cocktail.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
JCB117 + HM47/A9
Applications
WB, FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD79a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD79a antibody is available for research use only.
Storage Conditions
Store the CD79a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell surface, cytoplasmic
Image Legend
IHC testing of FFPE human tonsil (10X) stained with CD79a antibody cocktail (clone JCB117 + HM47/A9) .

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