CD68 Antibody

• Description:

  This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. antibody to CD68 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. CD68 antibody reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. CD68 shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

• Specifications:

  Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat, Cat

• Immunogen:

  The subcellular fraction of human alveolar macrophages was used as the immunogen for this CD68 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  C68/684

• Applications:

  WB, IF, FACS, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD68 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD68 antibody is available for research use only.

• Storage Conditions:

  Store the CD68 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, membrane

• Image Legend:

  IHC testing of human tonsil (10X) stained with CD68 antibody (C68/684) .