CD34 Antibody

• Description:

  This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens) . Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

• Specifications:

  Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood, Immunofluorescence: 1:50-1:100

• UniProt:

  P28906

• Host:

  Mouse

• Reactivity:

  Human, Rat

• Immunogen:

  Blast cells from a chronic myeloid leukemia patient were used as the immunogen for this CD34 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  ICO-115

• Applications:

  FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  CF647 Conjugate

• Buffer:

  500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the CD34 antibody at 2-8oC, protected from light.

• Limitations:

  This CD34 antibody is available for research use only.

• Storage Conditions:

  Store the CD34 antibody at 2-8°C, protected from light.

• Formulation:

  500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD34 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  Surface flow cytometric analysis of CD34 on human KG-1 cells using CD34 antibody (clone ICO-115, red) and isotype control antibody (green) .