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CD28 Antibody

CAT: 0800-V2054SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V2054SAF-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
CAS Number
9007-83-4
Specifications
ELISA (order BSA/sodium azide-free format for coating)
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human CD28 protein was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
C28/77
Applications
ELISA
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the CD28 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD28 antibody is available for research use only.
Storage Conditions
Store the CD28 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes.
Location
Cell surface
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free CD28 Antibody (clone C28/77) . Confirmation of Integrity and Purity of the Antibody.
Product Datasheet
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