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PPP2R1A Antibody

CAT: 0800-RQ6671Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6671Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform is an enzyme that in humans is encoded by the PPP2R1A gene. This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL
UniProt
P30153
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Amino acids KVKEFCENLSADCRENVIMSQ from the human protein were used as the immunogen for the PPP2R1A antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, IHC-P
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the PPP2R1A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PPP2R1A antibody is available for research use only.
Storage Conditions
After reconstitution, the PPP2R1A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PPP2R1A antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
Immunofluorescent staining of FFPE human A549 cells with PPP2R1A antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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