ETFA Antibody

CAT: 0800-RQ6622Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-RQ6622Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The human ETFA gene encodes the Electron-transfer-flavoprotein, alpha subunit, also known as ETF-a. Together with Electron-transfer-flavoprotein, beta subunit, encoded by the 'ETFB' gene, it forms the heterodimeric Electron transfer flavoprotein (ETF) . ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P13804
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids M1-K333) was used as the immunogen for the ETFA antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the ETFA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ETFA antibody is available for research use only.
Storage Conditions
After reconstitution, the ETFA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ETFA antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human colonic adenocarcinoma tissue with ETFA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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