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ETF1 Antibody / eRF1

CAT: 0800-RQ6232Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6232Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Eukaryotic translation termination factor 1 (eRF1), also known as TB3-1, is a protein that in humans is encoded by the ETF1 gene. It is mapped to 5q31.2. This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD) . Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P62495
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant partial protein (amino acids D9-K342) was used as the immunogen for the ETF1 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
3E5.
Applications
WB, IHC-P, IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the ETF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ETF1 antibody is available for research use only.
Storage Conditions
After reconstitution, the ETF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ETF1 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of rat 1) liver, 2) testis, 3) kidney, 4) PC-12, and mouse 5) liver, 6) testis, 7) kidney and 8) RAW264.7 cell lysate with ETF1 antibody. Predicted molecular weight ~49 kDa.

UniProtKB · P62495

Eukaryotic peptide chain release factor subunit 1

ERF1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P62495
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ETF1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Eukaryotic release factor 1, eRF1
EC number
—
Processing
—
Secondary accessions
B2R6B4, D3DQC1, P46055, Q5M7Z7, Q96CG1
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationHydroxylationIsopeptide bondMethylationPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Cellular component

Cytoplasm

Biological process

Nonsense-mediated mRNA decayProtein biosynthesis

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