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PPIE Antibody / Cyclophilin E

CAT: 0800-RQ6585Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6585Size:100 µg
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Description
Peptidylprolyl isomerase E (cyclophilin E), also known as PPIE, is an enzyme which in humans is encoded by the PPIE gene on chromosome 1. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
Q9UNP9
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids M1-V301) was used as the immunogen for the PPIE antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
7F2
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the PPIE antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PPIE antibody is available for research use only.
Storage Conditions
After reconstitution, the PPIE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PPIE antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of 1) rat heart, 2) rat brain, 3) rat lung, 4) mouse heart, 5) mouse brain, 6) mouse lung, 7) rat RH35 and 8) mouse HEPA1-6 cell lysate with PPIE antibody. Predicted molecular weight ~33 kDa.

UniProtKB · Q9UNP9

Peptidyl-prolyl cis-trans isomerase E

PPIE_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9UNP9
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PPIE
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PPIase E
EC number
5.2.1.8
Processing
—
Secondary accessions
B2R971, O43634, O43635, Q32Q72, Q3S611, Q5TGA0, Q5TGA2, Q5TGA3, Q9UIZ5
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

IsomeraseRNA-bindingRotamase

Biological process

mRNA processingmRNA splicing

Cellular component

NucleusSpliceosome

PTM

Phosphoprotein

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