COPS5 Antibody / JAB1

• Description:

  COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis), also known as COPS5 or JAB1, is a gene conserved from humans to Saccharomyces cerevisiae. It is a member of the MOV34 family. COPS5 is mapped to 8q13.1. The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. COPS5 can interact with the cytoplasmic domain of the beta-2 subunit of the alpha-L/beta-2 integrin LFA1, and it is the only protein demonstrated to interact with MIF. COPS5, VHL, and TRC8 proteins appear to be linked both physically and functionally, and all 3 may participate in the development of kidney cancer. In addition to that, COPS5 is an essential cofactor for the apoptotic function of E2F1.

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  Q92905

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  An E. coli-derived human protein (amino acids M8-S334) was used as the immunogen for the COPS5 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  4G9

• Applications:

  WB, IHC-P, IF, FACS

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the COPS5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This COPS5 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the COPS5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the COPS5 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear and cytoplasmic

• Image Legend:

  IHC staining of FFPE human liver cancer tissue with COPS5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.