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Ki-67 Antibody

CAT: 0800-RQ6529Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6529Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P46013
Host
Mouse
Reactivity
Human
Immunogen
An E. coli-derived human protein (amino acids K2860-I3256) was used as the immunogen for the Ki-67 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
5E12.
Applications
IHC-P, IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Ki-67 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Ki-67 antibody is available for research use only.
Storage Conditions
After reconstitution, the Ki-67 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Ki-67 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Immunofluorescent staining of FFPE human tonsil tissue with Ki-67 antibody (green) . HIER: steam section in pH6 citrate buffer for 20 min.
Product Datasheet
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