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TRAP alpha Antibody / TRAPA / SSR1

CAT: 0800-RQ6522Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6522Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Translocon-associated protein subunit alpha is a protein that in humans is encoded by the SSR1 gene. The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. Multiple transcript variants encoding different isoforms have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P43307
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
An E. coli-derived human protein (amino acids E53-E286) was used as the immunogen for the TRAP alpha antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the TRAP alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This TRAP alpha antibody is available for research use only.
Storage Conditions
After reconstitution, the TRAP alpha antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the TRAP alpha antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with TRAP alpha antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · P43307

Translocon-associated protein subunit alpha

SSRA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P43307
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SSR1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
TRAP-alpha
EC number
—
Processing
Precursor
Secondary accessions
A8K685, Q53GX2, Q53H19, Q5TAM3, Q6IB43, Q8NBH9, Q96IA2, Q9TNQ8, Q9UN49
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

Calcium

Cellular component

Endoplasmic reticulumMembrane

PTM

GlycoproteinPhosphoprotein

Domain

SignalTransmembraneTransmembrane helix

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