Neurofilament heavy Antibody / Nefh

• Description:

  Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  P19246

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  A mouse recombinant partial protein (amino acids Y109-E466) was used as the immunogen for the Neurofilament heavy antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, FACS, Direct ELISA

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the Neurofilament heavy antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Neurofilament heavy antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Neurofilament heavy antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Neurofilament heavy antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  IHC staining of FFPE mouse spinal cord with Neurofilament heavy antibody (right) and negative control (left) . HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.