EIF4A1 Antibody

• Description :

  Eukaryotic initiation factor 4A-I is a protein that in humans is encoded by the EIF4A1 gene. It is mapped to 17p13.1. EIF4A1 has been shown to interact with EIF4E and eukaryotic translation initiation factor 4 gamma.

• Specifications :

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt :

  P60842

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Amino acids LQQIELDLKATQALVLA from the human protein were used as the immunogen for the EIF4A1 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b

• Clone :

  11B8

• Applications :

  WB, IHC-P, IF, FACS

• Purity :

  Affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution :

  After reconstitution, the EIF4A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This EIF4A1 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the EIF4A1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the EIF4A1 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic

• Image Legend :

  Immunofluorescent staining of FFPE human Caco-2 cells with EIF4A1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.