EIF4A2 Antibody

CAT: 0800-F54470-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
CAT#:0800-F54470-0.08MLSize:0.08 mL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice; yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation (referenced from OMIM) .
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25
UniProt
Q14240
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 333-360 from the human protein was used as the immunogen for the EIF4A2 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, FACS, IHC-P
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the EIF4A2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This EIF4A2 antibody is available for research use only.
Storage Conditions
Aliquot the EIF4A2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the EIF4A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC testing of FFPE human lung carcinoma tissue with EIF4A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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