ICAD Antibody

• Description:

  DNA fragmentation factor subunit alpha (DFFA), also known as Inhibitor of caspase-activated DNase (ICAD), is a protein that in humans is encoded by the DFFA gene. Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

• Specifications:

  Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  O00273

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  A human recombinant partial protein (amino acids E14-S308) was used as the immunogen for the ICAD antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS, Direct ELISA

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.0125% sodium azide

• Reconstitution:

  After reconstitution, the ICAD antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This ICAD antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the ICAD antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the ICAD antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Flow cytometry testing of human A431 cells with ICAD antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= ICAD antibody.