RAPGEF2 Antibody

• Description :

  Rap guanine nucleotide exchange factor 2 is a protein that in humans is encoded by the RAPGEF2 gene. Members of the RAS subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation.

• Specifications :

  Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt :

  Q9Y4G8

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A human recombinant partial protein (amino acids S1266-V1499) was used as the immunogen for the RAPGEF2 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, FACS, Direct ELISA

• Purity :

  Affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the RAPGEF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This RAPGEF2 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the RAPGEF2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the RAPGEF2 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Image Legend :

  Flow cytometry testing of human HeLa cells with RAPGEF2 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= RAPGEF2 antibody.