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NDUFB2 Antibody

CAT: 0800-RQ6144Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6144Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial is an enzyme that in humans is encoded by the NDUFB2 gene. The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I) . Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays a important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Hydropathy analysis revealed that this subunit and 4 other subunits have an overall hydrophilic pattern, even though they are found within the hydrophobic protein (HP) fraction of complex I.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
O95178
Host
Rabbit
Reactivity
Human
Immunogen
A human recombinant partial protein (amino acids R16-D105) was used as the immunogen for the NDUFB2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the NDUFB2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NDUFB2 antibody is available for research use only.
Storage Conditions
After reconstitution, the NDUFB2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the NDUFB2 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human lung cancer with NDUFB2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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