HSD17B2 Antibody

• Description :

  HSD17B2 (17-BETA-HYDROXYSTEROID DEHYDROGENASE II), also called 17-BETA-HSD II, is an anzyme which have 387 amino acids with a predicted molecular weight of 42,782 and associate with the membranes of the endoplasmic reticulum. Its Cytogenetic location is 16q23.3. The type 2 enzyme was capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. HSD17B2 mRNA was detected in 18 of 42 (43%) adenomas but not in prolactinomas. In the human endometrium, inactivation of 17-beta-estradiol to estrone is catalyzed by HSD17B2. And HSD17B2 activity distinguishing between disease-free and diseased endometria. HSD17B2 efficiently catalyzes the oxidative metabolism of androgens and estrogens, and it is expressed in a large series of human peripheral tissues. The previous paradigm that HSD17B2 activity in the endometrium is elevated during the secretory phase is confined to diseased endometrium but not to disease-free endometrium.

• Specifications :

  Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt :

  P37059

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Amino acids IYDYFAKRHFGQDK from the human protein were used as the immunogen for the HSD17B2 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, FACS

• Purity :

  Affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the HSD17B2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This HSD17B2 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the HSD17B2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the HSD17B2 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Image Legend :

  Flow cytometry testing of human Caco-2 cells with HSD17B2 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= HSD17B2 antibody.