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OCLN Antibody / Occludin

CAT: 0800-RQ5746Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5746Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Occludin is an integral membrane protein that is located at tight junctions. It is a member of a family of proteins containing the highly conserved Marvel domain, which contains 4 transmembrane-helix regions. This gene is mapped to 5q13.2. Occludin regulates TGF-beta receptor type I localization for efficient TGF-beta-dependent dissolution of tight junctions during epithelial-mesenchymal transitions. Human Occludin is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins. It has been found that occludin is involved in cell migration and functions to recruit active PI3 kinase to the leading edge, resulting in RAC1 activation and formation of lamellipodia.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q16625
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human protein (amino acids H54-K270) was used as the immunogen for the OCLN antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the OCLN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This OCLN antibody is available for research use only.
Storage Conditions
After reconstitution, the OCLN antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the OCLN antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human A431 cells with OCLN antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · Q16625

Occludin

OCLN_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q16625
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
OCLN
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B5BU70, D2DU64, D2DU65, D2IGC0, D2IGC1, E2CYV9, Q5U1V4, Q8N6K1
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell junctionCell membraneMembraneTight junction

Domain

Coiled coilTransmembraneTransmembrane helix

Disease

Disease variant

PTM

Disulfide bondPhosphoprotein

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