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PRMT5 Antibody

CAT: 0800-RQ5621Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5621Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Protein arginine N-methyltransferase 5 is an enzyme that in humans is encoded by the PRMT5 gene. It is mapped to 14q11.2. This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
O14744
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant protein (amino acids R18-A552) was used as the immunogen for the PRMT5 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the PRMT5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PRMT5 antibody is available for research use only.
Storage Conditions
After reconstitution, the PRMT5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PRMT5 antibody should be determined by the researcher.
Image Legend
Flow cytometry testing of human 293T cells with PRMT5 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= PRMT5 antibody.

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