IQGAP1 Antibody

CAT: 0800-RQ5586Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-RQ5586Size:100 µg
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Description
Ras GTPase-activating-like protein (IQGAP1) also known as p195 is a ubiquitously expressed protein that in humans is encoded by the IQGAP1 gene. It is mapped to 15q26.1. This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.
CAS Number
9007-83-4
Specifications
Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P46940
Host
Rabbit
Reactivity
Human
Immunogen
A human recombinant protein (amino acids S2-H578) was used as the immunogen for the IQGAP1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, IF, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the IQGAP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This IQGAP1 antibody is available for research use only.
Storage Conditions
After reconstitution, the IQGAP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the IQGAP1 antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear, membrane
Image Legend
Immunofluorescent staining of human A431 cells with IQGAP1 antibody (green) and DAPI nuclear stain (blue) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

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