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Orai1 Antibody

CAT: 0800-RQ5543Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5543Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ORAI1 (ORAI calcium release-activated calcium modulator 1), also known as CRACM1, Calcium release-activated calcium channel protein 1 and TMEM142A, is a calcium selectiveion channel that in humans is encoded by the ORAI1 gene. Orai1 channels play an important role in the activation of T-lymphocytes. The loss of function mutation of Orai1 causes severe combined immunodeficiency (SCID) in humans. The mammalian orai family has two additional homologs, orai2 and orai3. Orai proteins share no homology with any other ion channel family of any other known proteins. They have 4 transmembrane domains and form tetramers. Prakriya et al. showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments. Glu106-to-asp (E106D) and glu190-to-gln (E190Q) substitutions in transmembrane helices 1 and 3, respectively, diminished calcium ion influx, increased current carried by monovalent cations, and rendered the channel permeable to cesium ion.Prakriya et al. showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments.
CAS Number
9007-83-4
Specifications
Western blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q96D31
Host
Rabbit
Reactivity
Human
Immunogen
A human recombinant protein (amino acids A49-A301) was used as the immunogen for the Orai1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Orai1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Orai1 antibody is available for research use only.
Storage Conditions
After reconstitution, the Orai1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Orai1 antibody should be determined by the researcher.
Image Legend
Western blot testing of human 1) A375, 2) Jurkat, 3) A549, 4) A431, 5) HepG2 and 6) K562 cell lysate with Orai1 antibody. Predicted molecular weight: 33-50 kDa depending on glycosylation level.
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