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Nucleolin Antibody

CAT: 0800-RQ5539Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5539Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Nucleolin is a protein that in humans is encoded by the NCL gene. It is mapped to 2q37.1. Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
CAS Number
9007-83-4
Specifications
Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 2-4 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P19338
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
A human recombinant protein (amino acids K219-A629) was used as the immunogen for the Nucleolin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, IF, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Nucleolin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Nucleolin antibody is available for research use only.
Storage Conditions
After reconstitution, the Nucleolin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Nucleolin antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of human HepG2 cell lysate with Nucleolin antibody. Predicted molecular weight ~77 kDa, commonly observed at 100~110 kDa.

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