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NRF1 Antibody

CAT: 0800-RQ5524Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5524Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Nuclear respiratory factor 1, is also known as NRF1. This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for nuclear factor (erythroid-derived 2) -like 1 which has an official symbol of NFE2L1.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 2-4 µg/mL
UniProt
Q16656
Host
Mouse
Reactivity
Human
Immunogen
A human recombinant protein (amino acids D246-Q503) was used as the immunogen for the NRF1 antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
2G4
Applications
WB, IHC-P, IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the NRF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NRF1 antibody is available for research use only.
Storage Conditions
After reconstitution, the NRF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the NRF1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
IHC staining of FFPE human testis cancer with NRF1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Product Datasheet
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