NRF1 Antibody

• Description :

  Nuclear respiratory factor 1, is also known as NRF1. This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for nuclear factor (erythroid-derived 2) -like 1 which has an official symbol of NFE2L1.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 2-4 µg/mL

• UniProt :

  Q16656

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A human recombinant protein (amino acids D246-Q503) was used as the immunogen for the NRF1 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2a

• Clone :

  2G4

• Applications :

  WB, IHC-P, IF, FACS

• Purity :

  Affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the NRF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This NRF1 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the NRF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the NRF1 antibody should be determined by the researcher.

• Location :

  Nuclear

• Image Legend :

  IHC staining of FFPE human testis cancer with NRF1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.