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PAX5 Antibody

CAT: 0800-RQ5521Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5521Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Paired box protein Pax-5 is a protein that in humans is encoded by the PAX5 gene. The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding domain, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL
UniProt
Q02548
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant protein (amino acids R217-A282) was used as the immunogen for the PAX5 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
6I4
Applications
WB, IF, IHC-P
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the PAX5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PAX5 antibody is available for research use only.
Storage Conditions
After reconstitution, the PAX5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PAX5 antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with PAX5 antibody (green) and DAPI (blue) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Product Datasheet
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