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SAE2 Antibody

CAT: 0800-RQ5505Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5505Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ubiquitin-like 1-activating enzyme E1B (UBLE1B) also known as SUMO-activating enzyme subunit 2 (SAE2) is an enzyme that in humans is encoded by the UBA2 gene. Posttranslational modification of proteins by the addition of the small protein SUMO, or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
Q9UBT2
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant protein (amino acids E449-K564) was used as the immunogen for the SAE2 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
5H11
Applications
WB, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the SAE2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This SAE2 antibody is available for research use only.
Storage Conditions
After reconstitution, the SAE2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the SAE2 antibody should be determined by the researcher.
Image Legend
Flow cytometry testing of human A431 cells with SAE2 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= SAE2 antibody.

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