SUMO-activating enzyme subunit 1 Antibody / SAE1

• Description:

  The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.

• Specifications:

  Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:25

• UniProt:

  Q9UBE0

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 300-329 from the human protein was used as the immunogen for the SUMO-activating enzyme subunit 1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC-P

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the SUMO-activating enzyme subunit 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This SUMO-activating enzyme subunit 1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the SUMO-activating enzyme subunit 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the SUMO-activating enzyme subunit 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Nuclear, cytoplasmic

• Image Legend:

  Western blot testing of human HEK293 cell lysate with SUMO-activating enzyme subunit 1 antibody. Predicted molecular weight ~38 kDa.