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SFRP1 Antibody

CAT: 0800-RQ4938Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4938Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Secreted frizzled-related protein 1, also known as SFRP1, is a protein which in humans is encoded by the SFRP1 gene. It is mapped to 8p11.21. This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q8N474
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids A204-K314 from the human protein were used as the immunogen for the SFRP1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the SFRP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This SFRP1 antibody is available for research use only.
Storage Conditions
After reconstitution, the SFRP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the SFRP1 antibody should be determined by the researcher.
Location
Cytoplasmic, secreted
Image Legend
Western blot testing of 1) human A549, 2) human U-2 OS, 3) human PC-3, 4) rat heart, 5) mouse heart and 6) mouse HEPA1-6 lysate wtih SFRP1 antibody at 0.5ug/ml. Predicted molecular weight ~35 kDa.

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