TRX Antibody / Thioredoxin / TXN

• Description:

  Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunocytochemistry (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (mouse recombinant protein), Immunofluorescence: 2-4 µg/mL

• UniProt:

  P10639

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  Amino acids V2-A105 from the mouse protein were used as the immunogen for the TRX antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, ICC, Direct ELISA, IF

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the TRX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This TRX antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the TRX antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the TRX antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear, cytoplasmic

• Image Legend:

  Western blot testing of mouse 1) testis, 2) kidney, 3) small intestine, 4) spleen, 5) stomach, 6) lung, 7) smooth muscle and 8) heart lysate with TXN antibody at 0.5ug/ml. Predicted molecular weight ~12 kDa.