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Hsp27 Antibody

CAT: 0800-RQ4574Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4574Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HSPB1 (Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. It is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN) .
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 2-3 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (recombinant mouse protein)
UniProt
P14602
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
Amino acids M1-K209 from the mouse protein were used as the immunogen for the Hsp27 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Hsp27 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Storage Conditions
After reconstitution, the Hsp27 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Hsp27 antibody should be determined by the researcher.
Location
Predominantly cytoplasmic with some nuclear
Image Legend
Western blot testing of 1) rat skeletal muscle, 2) rat lung and mouse 3) spleen, 4) skeletal muscle, 5) lung, 6) liver and 7) testis lysate with Hsp27 antibody at 0.5ug/ml. Predicted molecular weight: 23-27 kDa.

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