Dynamin 2 Antibody / DNM2

• Description:

  Dynamin-2 is a protein that in humans is encoded by the DNM2 gene. Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined.

• Specifications:

  Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  P50570

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  A recombinant human protein corresponding to amino acids A177-Q441 was used as the immunogen for the Dynamin 2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the Dynamin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Dynamin 2 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Dynamin 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Dynamin 2 antibody should be determined by the researcher.

• Prediction Reactivity:

  Human

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm, cytoskeleton, cell junction

• Image Legend:

  Western blot testing of 1) rat brain and 2) mouse brain lysate with Dynamin 2 antibody at 0.5ug/ml. Predicted molecular weight ~98 kDa.