Zebrafish Dnm1 Antibody / Dnm1a / Dnm1b / Dynamin

• Description:

  Zebrafish express multiple dnm1 transcript variants that give rise to isoform a and isoform b, which may differ in their tissue distribution, regulatory elements, or functional properties. Both isoforms contribute to the dynamic regulation of synaptic membranes but may be expressed at different developmental stages or in distinct neuronal populations. Dnm1 isoform a is typically expressed during early neural development and is enriched in regions of the brain with high synaptic activity. It supports the rapid and repeated cycles of vesicle fission that are essential for sustained neurotransmitter release. Dnm1 isoform b may have overlapping or complementary roles to isoform a, potentially contributing to synaptic maintenance or plasticity in mature neurons. It may also be involved in neural differentiation and axonal growth during zebrafish development. Because of its essential function in synaptic physiology, zebrafish Dnm1 is a widely used model for studying neural development, synaptic transmission, and neurological disorders associated with defects in vesicle recycling. It is particularly valuable in research on seizure susceptibility and developmental brain disorders, where Dnm1 mutations are known to have pathogenic effects in other vertebrates.

• Specifications:

  Immunofluorescence: 5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

• UniProt:

  A0A8M2BG30, A0A8M2BCU8

• Host:

  Rabbit

• Reactivity:

  Zebrafish

• Immunogen:

  E. coli-derived zebrafish Dnm1a/b recombinant protein (amino acids G614-D666) was used as the immunogen for the Zebrafish Dnm1 antibody. This antibody will detect the a & b isoforms.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IF, IHC-P

• Purity:

  Antigen affinity chromatography

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Limitations:

  This Zebrafish Dnm1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Zebrafish Dnm1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Zebrafish Dnm1 antibody should be determined by the researcher.

• Location:

  Cytoplasm

• Image Legend:

  IHC staining of FFPE zebrafish brain tissue with Zebrafish Dnm1 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.