Cullin 5 Antibody / CUL5

• Description:

  Cullin-5 is a protein that in humans is encoded by the CUL5 gene. Cul5 inhibits cellular proliferation, potentially through its involvement in the SOCS/ BC-box/ eloBC/ cul5/ RING E3 ligase complex, which functions as part of the ubiquitin system for protein degradation. One study have shown that Cul5 plays a role in Reelin signaling cascade, participating in the DAB1 degradation and thus ensuring the negative feedback mechanism of Reelin signaling during corticogenesis.

• Specifications:

  Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL (human recombinant protein)

• UniProt:

  Q93034

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A recombinant human protein corresponding to amino acids A2-D105 was used as the immunogen for the Cullin 5 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the Cullin 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Cullin 5 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Cullin 5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Cullin 5 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of rat 1) brain, 2) spleen, 3) heart, 4) kidney and mouse 5) brain, 6) spleen, 7) heart, 8) kidney and 9) testis lysate with Cullin 5 antibody at 0.5ug/ml. Predicted molecular weight ~91 kDa.