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NEDD4-2 Antibody / NEDD4L

CAT: 0800-RQ4197Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4197Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Neural precursor cell expressed developmentally downregulated gene 4-like (NEDD4L) or NEDD4-2 (NEDD4-2) is an enzyme (ubiquitin ligase) of the NEDD4 family. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, FACS: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q96PU5
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A recombinant human protein corresponding to amino acids N703-D975 was used as the immunogen for the NEDD4-2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the NEDD4-2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NEDD4-2 antibody is available for research use only.
Storage Conditions
After reconstitution, the NEDD4-2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the NEDD4-2 antibody should be determined by the researcher.
Image Legend
Western blot testing of 1) human 22RV1, 2) human Jurkat, 3) rat pancreas, 4) mouse pancreas and 5) mouse NIH3T3 lysate with NEDD4-2 antibody at 0.5ug/ml. Predicted molecular weight: 112-125 kDa.

UniProtKB · Q96PU5

E3 ubiquitin-protein ligase NEDD4-like

NED4L_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q96PU5
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NEDD4L
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.3.2.26, 2.3.2.36
Processing
—
Secondary accessions
O43165, Q3LSM7, Q7Z5F1, Q7Z5F2, Q7Z5N3, Q8N5A7, Q8WUU9, Q9BW58, Q9H2W4, Q9NT88
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmEndosomeGolgi apparatus

Biological process

DifferentiationHost-virus interactionUbl conjugation pathway

Disease

Disease variant

Domain

Repeat

Molecular function

Transferase
Product Datasheet
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