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Bid Antibody

CAT: 0800-R32856Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32856Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, the human BID gene is mapped to 22q11. It is reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P70444
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
A recombinant mouse protein corresponding to amino acids M1-D195 was used as the immunogen for the Bid antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Reconstitution
After reconstitution, the Bid antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Bid antibody is available for research use only.
Storage Conditions
After reconstitution, the Bid antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Bid antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of mouse brain tissue with Bid antibody at 0.5ug/ml. Predicted molecular weight ~22 kDa.

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