Flt3 Antibody / CD135

• Description:

  Cluster of differentiation antigen 135 (CD135) also known as fms like tyrosine kinase 3 (FLT-3), receptor-type tyrosine-protein kinase FLT3, or fetal liver kinase-2 (Flk2) is a protein that in humans is encoded by the FLT3 gene. This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow.

• Specifications:

  Western Blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA (mouse recombinant protein) : 0.1-0.5 µg/mL (BSA-free formulation available)

• UniProt:

  Q00342

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  A recombinant mouse protein corresponding to amino acids E62-E295 was used as the immunogen for the Flt3 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS, Direct ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the Flt3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Flt3 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Flt3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Flt3 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell membrane, cytoplasm

• Image Legend:

  Western blot testing of mouse brain lysate with Flt3 antibody at 0.5ug/ml. Predicted molecular weight: ~113 kDa (unmodified), ~130/160 kDa (glycosylated) .