CD135 Antibody / FLT3

• Description:

  Stem cell tyrosine kinase (STK-1) has been cloned from a CD34+ hematopoietic stem cell enriched library and identified as the human homolog of a previously identified gene of Mouse origin designated either Flk-2 or Flt-3. The STK-1 cDNA encodes a protein of 993 amino acids with 85% identity to Flt-3/Flk-2. STK-1 is a member of the type III receptor tyrosine kinase family that includes Kit (steel factor receptor), Fms and PDGF. STK-1 expression in blood and marrow is restricted to CD34+ cells, a population greatly enriched for hematopoietic stem/progenitor cells. STK-1 antiserum recognizes two polypeptides in these cells. The Mouse homolog of STK-1, designated Flt-3/Flk-2, is expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues. It has been suggested that STK-1 and its murine homolog Flt-3/Flk-2 may function as growth factor receptors on hematopoietic stem and/or progenitor cells.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P36888

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 500-800 from human FLT3 protein was used as the immunogen for the CD135 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  FLT3/9888

• Applications:

  IHC-P

• Purity:

  Protein G affinity

• Format:

  Purified

• Limitations:

  This CD135 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD135 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD135 antibody should be determined by the researcher.

• Location:

  Endoplasmic reticulum lumen, Membrane

• Image Legend:

  IHC staining of FFPE human brain tissue with CD135 antibody (clone FLT3/9888) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.