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Hemoglobin Antibody (Subunit alpha)

CAT: 0800-R32748Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32748Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some non-deletion alpha thalassemias have also been reported.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P69905
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids V2-R142 from the human alpha subunit were used as the immunogen for the Hemoglobin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Hemoglobin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Hemoglobin antibody is available for research use only.
Storage Conditions
After reconstitution, the Hemoglobin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Hemoglobin antibody should be determined by the researcher.
Image Legend
IHC testing of FFPE human placental tissue with Hemoglobin antibody. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to testing.

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