EPHX2 Antibody

• Description:

  Soluble epoxide hydrolase (sEH), or Epoxide hydrolase 2, is a bifunctional enzyme that in humans is encoded by the EPHX2 gene. It is mapped to 8p21.2-p21.1. This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described.

• Specifications:

  Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/10^6 cells

• Gene ID:

  2053

• UniProt:

  P34913

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Amino acids 505-543 (QHMEDWIPHLKRGHIEDCGHWTQMDKPTEVNQILIKWLD-human) were used as the immunogen for the EPHX2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the EPHX2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This EPHX2 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the EPHX2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the EPHX2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of 1) rat lung, 2) mouse lung and 3) HepG2 lysate with EPHX2 antibody at 0.5ug/ml. Predicted molecular weight: ~63/57 kDa (isoforms 1/2) .