ABCE1 Antibody

• Description :

  ATP binding cassette E1 (ABCE1, also called RNase L inhibitor) is an ATPase found in humans involved in viral assembly. It is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily. ABCE1 inhibits the action of ribonuclease L. Ribonuclease L normally binds to 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) and inhibits the interferon-regulated 2-5A/RNase L pathway, which is used by viruses. ABCE1 heterodimerize with ribonuclease L and prevents its interaction with 2-5A, antagonizing the anti-viral properties of ribonuclease L, and allow the virus to synthesize viral proteins. It has also been implicated to have an effect in tumorcell proliferation and antiapoptosis.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2ug antibody/500ug lysate, Flow cytometry: 1-3ug/million cells

• UniProt :

  P61221

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Amino acids K419-D599 from the human protein were used as the immunogen for the ABCE1 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IF, IP, FACS

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution :

  After reconstitution, the ABCE1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This ABCE1 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the ABCE1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Differences in protocols and secondary/substrate sensitivity may require the ABCE1 antibody to be titrated for optimal performance.

• Location :

  Cytoplasmic

• Image Legend :

  Immunofluorescent staining of FFPE human HeLa cells with ABCE1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.